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Twenty-five colonies were initiated into the experiment on 5-Oct-2010 and 23 more on 12-Oct-2010. We first transferred 50 haphazardly selected workers and the queen into new containers (cylindrical, ∼10 cm in radius, 10 cm in height). Some colonies had matured gynes that had lost their wings; these could not be reliably distinguished from the founding queen and thus were included in the transfer. We discounted one worker for each additional gyne. We initially provided the colonies with two nesting tubes; these were replaced as they dried or became moldy. Three times a week, we fed each colony 0.2±0.02 g of standardized diet and removed food remaining from the previous feeding.

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REFERENCE: “Pacific and Atlantic Herring Produce Burst Pulse Sounds,” Ben Wilson, Robert S. Batty and Lawrence M. Dill, Biology Letters, vol. 271, 2003, pp. S95-S97.